Cell viability & cell proliferation

Flow cytometry allows to analyse cell viability. This analysis will be performed by the researcher or by a member of the platform staff, on the FACSCanto II, or on the FACSFortessa or on the FACSCalibur or on the FACSVerse.

In general, it is usefull to have a discussion before the beginning of the experiment, to ensure a proper preparation of the samples.

 

Access

These equipment can be used by researchers themselves. Before any access to the equipment, it is however mandatory to :

  • read the platform regulation and to accept its terms
  • fill the Biosafety Level 1 form (Français/English) before any new experiment
  • follow a training: all users must be trained by the platform coordinator, not by other users. If you are already experienced in using such instrument, the coordinator will supervised your first session to verify that you are able to handle the instrument correctly and according to the local oparating procedures.
The use of the cytometers is scheduled on the GIGA intranet.
 

Software

The softwares for cytometry CellQuest Pro, FACSDiva, FACSuite or FlowJo are available on computers that are independant from the cytometers (in the data room). Computers piloting flow cytometers have to be used in prioritory for data acquisition and not for data analysis.
 

Delivrable

Raw data and/or data analysed with softawres for cytometry have to be saved and exported by the costumer after each data acquisition and/or analysis. A printer is also available to print the data out. Long-term storage is not performed by the facility and has to be done by the costumer himself.
 

Contact

If you are interested, contact  Sandra Ormenese or Raafat Stephan
 

Questions

If you have any questions, refer to the FAQs or mail to imaging.giga@ulg.ac.be