Cell sorting and cloning will exclusively be performed on the FACSAria III u or the FACSAria III cytometers by Sandra Ormenese, in charge of the facility or Raafat Stephan, the flow cytometry specialist of the facility.
The FACSAria III u and the FACSAria III offer the possibility to sort safely potentially infected cells such as unfixed human cells or cells infected by virus into four sub-populations.
It is possible to work in sterile conditions on both cytometers and to do cell cloning on 96-well plates.
It is mandatory to have a discussion with Sandra Ormenese or Raafat Stephan before the beginning of the experiment to choose between the two FACS cell sorters and to assure a proper preparation of the samples.
Sorted cells are directly available. The composition of the suspension will be analysed and printed.
If you have any questions, refer to the FAQs or mail to firstname.lastname@example.org