In order to produce viral vectors, high quality plasmids are needed.
Our platform offers a maxiprep service as well as plasmid transformation into bacteria.
We use PureYield™ Plasmid Maxiprep System system (from Promega) in order to obtain transfection-quality plasmid DNA. This system provides a rapid method for purification and incorporates Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation. Purification is achieved without isopropanol precipitation of purified plasmid DNA.
We guarantee a minimum of 1 mL of purified plasmid (endotoxin-free and mycoplasma free) with a minimal concentration of 350 ng/µL. The integrity of all amplified plasmids is checked on agarose gel.