Studies with the zebrabox
The Zebrabox is an automated video tracking system that provides high throughput behavorial analysis data.
Some compounds (in toxicological assays for instance) or genetic defects can significantly alter behavior of zebrafish larvae when exposed to external stimuli such light or dark phases, temperature variations, vibration and sound. The Zebrabox is a great tool to model brain disorders (such as epilepsy, autism, schizophrenia) and locomoter deficits in several fields of research.
The ZebraBox includes:
- A video system to record the behaviour on up to 96 larvae (high resolution camera, lens)
- A lighting system to trigger night On/Off,
- An infrared illumination for steady lighting,
- High resolution and high frame rate recording,
- System Scalable to any plate : 6, 12, 24, 48 and 96 well plates.
The Zebrabox allows capturing motion of individual larvae placed in a 96-well microplate, or group motion in a Petri dish. The software analyses motion time and speed, and can draw the path that larvae swim.
The zebrafish larvae activity can be modulated by light / dark period, and by drugs.The ZebraLab software enables to automate this task and various experiment involving movement of organs (such as the gut transit or blood flow).
The system lets you load the videos you have acquired from your microscope. After selecting the zone where the heart is moving, the software will calculate the heart beat for each period of time. Several fish may be placed under the camera to allow medium throughput analysis.
An increasing number of high quality, peer-reviewed papers concerning behaviour and conditioning have been published recently.
You can find more information on this system on http://www.vplsi.com