CRISPR mutants and morphants generation
The zebrafish is a powerful tool for in vivo functional characterization of gene function and validation of candidate genomic sequences.
Until recently most loss-of-function studies in zebrafish have heavily relied on knocking-down target gene expression via morpholinos. By binding to complementary RNA, morpholinos antisense oligomers have been shown to be effective in knocking down some genes up to 5 days post-fertilization when injected into one cell stage embryos. Although MO knocking down continue to be a valuable tool for the field it has also obvious limitations such as a lack of heritability of the morphants as well as by causing some non-specific off target effects.
To have an unambiguous inside of the phenotype, some researchers prefer technologies allowing the generation of mutants. Given the ease of use of current site-specific nuclease technologies, the CRISPR system has demonstrated unprecedented efficacy and facility in enabling targeted gene disruption in zebrafish.
We offer both services: morpholinos provide, in principle, and accessible and straightforward method for gene knockdown in zebrafish.
For more information, to discuss your needs, or for a quote, please contact us.